What is rapid sequencing

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The use, distribution or reproduction in other forums is permitted, provided the original author s and the copyright owner s are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. You will fiind everything you need to discover our products, the technlogy and how it is being used around the world. The Rapid Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple two-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends.

Rapid Sequencing Adapters are then added to the tagged ends. The kit is optimised for simplicity and speed, rather than for obtaining maximum throughput.

Due to the simple nature of the workflow and the fact that little sample manipulation is required e. However, in order for long reads to be observed in sequencing, long fragments need to be present in the sample in the first place. Typical read length histogram when preparing an E. The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community. Addition of less than ng, or the presence of shorter fragments could compromise sequencing throughput and read length.

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Res Nurs Health. Download references. We are very grateful to all of the participants who took part in this study. We also gratefully acknowledge the contribution of Professor Maria Bitner-Glindzicz in the design and set-up of this study. You can also search for this author in PubMed Google Scholar. Correspondence to Melissa Hill. Reprints and Permissions. Hill, M. Delivering genome sequencing for rapid genetic diagnosis in critically ill children: parent and professional views, experiences and challenges.

Eur J Hum Genet 28, — Download citation. Received : 16 January Revised : 02 April Accepted : 12 May Published : 19 June Issue Date : November Anyone you share the following link with will be able to read this content:.

Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. European Journal of Human Genetics Genetics in Medicine Advanced search. Skip to main content Thank you for visiting nature. Download PDF. Subjects Health services Social sciences. Abstract Rapid genomic sequencing RGS is increasingly being used in the care of critically ill children. Recruitment Two participant groups were recruited: 1 Parents of children offered RGS as part of the RaPS study and 2 professionals genetic clinicians, non-genetic clinicians, genetic scientists and research consenters involved in offering and delivering RaPS.

Interviews Semi-structured interviews were conducted face-to-face or via telephone by researchers experienced in conducting interviews on sensitive topics MH, CL and JH. Table 1 Participant characteristics.